The Nutrient Requirements of Cells and Longevity System Rx™

In order to keep running, all living things need an unceasing supply of energy and materials. One useful way to discover the nutritional needs of cells is to attempt to culture them in the laboratory (in vitro). We shall examine the ingredients needed in the medium for culturing human cells. As this lists shows, it must make carbohydrates, proteins, DNA, and RNA from which they assemble cell membranes, ribosomes, mitochondria and so on.

NH4Cl and MgSO4 supply the nitrogen and sulfur atoms needed to synthesize proteins. Na2HPO4 and KH2PO4 supply the phosphorus atoms needed for nucleic acid synthesis (as well as being needed for the functioning of many proteins). These ingredients also supply the Na+, K+, Mg2+, and Cl- needed by the cell. CaCl2 supplies the needed Ca2+ ions. All these ingredients are dissolved in water and are taken up from this solution.

Ham's Medium:

This is the list of ingredients needed to grow human cells in culture (in vitro).

Ham's Tissue Culture Medium for Mammalian Cells
(amounts dissolved in 1 liter of triple distilled water)

L-Arginine 211 mg
Biotin 0.024 mg
L-Histidine 21 mg
Calcium pantothenate 0.7 mg
L-Lysine 29.3 mg
Choline chloride 0.69 mg
L-Methionine 4.48 mg
i-inositol 0.54 mg
L-Phenylalanine 4.96 mg
Niacinamide 0.6 mg
L-Tryptophan 0.6 mg
Pyridoxine hydrochloride 0.2 mg
L-Tyrosine 1.81 mg
Riboflavin 0.37 mg
L-Alanine 8.91 mg
Thymidine 0.7 mg
Glycine 7.51 mg
Cyanocobalamin 1.3 mg
L-Serine 10.5 mg
Sodium pyruvate 110 mg
L-Threonine 3.57 mg
Lipoic acid 0.2 mg
L-Aspartic acid 13.3 mg
CaCl 244 mg
L-Glutamic acid 14.7 mg
MgSO4 .7 mg
L-Asparagine 15 mg
Glucose 1.1 g
L-Glutamine 146.2 mg
NaC l7.4 g
L-Isoleucine 2.6 mg
KCl 285 mg
L-Leucine 13.1 mg
Na2HPO4 290 mg
L-Proline 11.5 mg
KH2PO 483 mg
L-Valine 3.5 mg
Phenol red 1.2 mg
L-Cysteine 31.5 mg
FeSO 40.83 mg
Thiamine hydrochloride 1 mg
CuSO4.5H2O 0.0025 mg
Hypoxanthine 4 mg
ZnSO4.7H2O 0.028 mg
Folic acid 1.3 mg
NaHCO3 1.2 g

This list includes:

All 20 of the amino acids from which proteins are synthesized; a purine (hypoxanthine) and a pyrimidine (thymidine) for the synthesis of nucleotides, and their polymers DNA and RNA; 2 precursors (choline and inositol) needed to synthesize some of the phospholipids in the cell; 8 vitamins, all of which serve as parts of coenzymes; the coenzyme lipoic acid; glucose as a source of energy and carbon atoms; the inorganic ions Na+, K+, Ca2+, Cu2+, Zn2+, and CO2+. Even when all of these ingredients have been mixed together, they still fail to grow (in vitro) unless some blood serum is added. It is assumed that just what metabolic needs are met by this supplement are trace amounts of hormones in the serum and are semingly critical. Why does a mammalian cell require such a complex broth compared to simple E. coli? It is the price of multicellularity. A mammal is made up of hundreds of different cell types; each specialized to function one or a few functions. All the many other functions of life - including the synthesis of many of the organic molecules it needs, it delegates to other cells. The extracellular fluid, derived from the blood, supplies it with these.

Our Longevity System Rx™ is scientifically developed to augment every context of Ham's medium and flood the body with the nutrients it needs for proper mitotic function and therefore synthesize the other compounds necessary to complete full protein synthesis.

Please contact us at customer service, toll free 888-AntiAging (268-4244) regarding the Longevity System Rx™ and its critical design.

Back to top Questions & Answers Back Home